mouse anti ruvbl2  (Santa Cruz Biotechnology)

Journal: Cell reports

Article Title: The Hao-Fountain syndrome protein USP7 regulates neuronal connectivity in the brain via a novel p53-independent ubiquitin signaling pathway

doi: 10.1016/j.celrep.2025.115231

Figure Lengend Snippet:

Article Snippet: Mouse anti-Ruvbl2 , Santa Cruz , Cat# sc-374135, RRID:AB_10915735.

Techniques: Western Blot, Immunoprecipitation, Immunofluorescence, Virus, Recombinant, Protease Inhibitor, Magnetic Beads, Mass Spectrometry, Software

Journal: Frontiers in Immunology

Article Title: RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation

doi: 10.3389/fimmu.2021.679184

Figure Lengend Snippet: RUVBL2 is essential for Nos2 gene expression and bactericidal activity of macrophages (A) Expression of Ruvbl2 in RAW264.7 cells. Cells were transfected with SMARTpool siRNA (SP-siRuvbl2), individual siRNA (siRuvbl2#1, siRuvbl2#2), or control siRNA (siControl) for 48 hours. Left, RT-PCR analysis of relative Ruvbl2 mRNA expression in response to different siRNA transfections. Right, Western blot analysis of RUVBL2 expression in cells transfected with siControl and SP-siRuvbl2 (upper panel), and with siControl, siRuvbl2#1 and siRuvbl2#2 respectively (lower panel). (B) Relative Nos2 mRNA expression in RAW 264.7 cells transfected with the corresponding siRNAs in the presence or absence of LPS (10 ng/ml) for 24 hours. (C) Level of nitrite in culture medium of RAW 264.7 cells transfected with the corresponding siRNAs in the presence or absence of LPS (10 ng/ml) for 24 hours. (D) Left, expression of Ruvbl1 gene in RAW 264.7 cells upon transfection with control siRNA (siRuvbl1) or siRNA against Ruvbl1 (siRuvbl1). Middle, level of Nos2 expression in RAW 264.7 cells transfected with the corresponding siRNAs in the presence or absence of LPS (10 ng/ml) for 24 hours. Right, level of nitrite in culture medium of RAW 264.7 cells transfected with the corresponding siRNAs in the presence or absence of LPS (10 ng/ml) for 24 hours. (E) RAW 264.7 cells transfected with siControl or SP-siRuvbl2 were infected with E.coli . The level of bacterial load (left) and nitrite levels in the corresponding condition medium after 24 h (right) were determined. (F) Western blotting analysis showing the expression of RUVBL2 in whole cell lysate (Upper panel), and subcellular fractions (Lower panel), upon LPS treatment for different time points. Data from (A–E) were obtained from three independent experiments and presented in mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 by one-way ANOVA with Bonferroni’s multiple comparison test as post-test in (A–C) and by unpaired t-test in (D, E) .

Article Snippet: For immunoblotting and ChIP assay, the following antibodies were used: mouse monoclonal anti-Reptin 52 (RUVBL2) (Santa Cruz), mouse anti-TIP49A (RUVBL1) (Abcam), mouse anti-β-Actin (Sigma-Aldrich), rabbit anti-p38/MAPK (Cell Signaling Technology), rabbit anti-p-p38/MAPK (T180/Y182; Cell Signaling Technology), rabbit anti-p44/42 (ERK1/2; Cell Signaling Technology), rabbit anti-p-p44/42 MAPK (ERK1/2; Thr202/Tyr204 (Cell Signaling Technology), rabbit anti-SAPK/JNK (Cell Signaling Technology), rabbit anti-p-SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology), rabbit anti-IκBα (Cell Signaling Technology), rabbit anti-Stat1 (Cell Signaling Technology), rabbit anti-p-Stat1(S727) (Cell Signaling Technology), rabbit anti-p-Akt (S473) (Cell Signaling Technology), mouse anti-LAMIN B1 (Santa Cruz), mouse anti-α-tubulin (Sigma-Aldrich), rabbit anit-p50 (Abcam), rabbit anti-H3K4Me3 (Cell Signaling Technology), rabbit anti-H4K20me3 (Santa Cruz Biotechnology), and rabbit anti-p50 (Cell Signaling Technology) antibodies.

Techniques: Gene Expression, Activity Assay, Expressing, Transfection, Control, Reverse Transcription Polymerase Chain Reaction, Western Blot, Infection, Comparison

Journal: Frontiers in Immunology

Article Title: RUVBL1/2 Complex Regulates Pro-Inflammatory Responses in Macrophages via Regulating Histone H3K4 Trimethylation

doi: 10.3389/fimmu.2021.679184

Figure Lengend Snippet: RUVBL2 is essential for pro-inflammatory gene expressions. (A) Heatmap of pro-inflammatory gene expression using real-time PCR analysis at 4 and 24 hrs after LPS induction of RAW 264.7 macrophages transfected with siControl or SP-siRuvbl2. (B) Level of TNF-α, IFNγ, IL-6, IL-1β, and GM-CSF in culture medium of RAW 264.7 macrophages transfected with siControl or siRuvbl2, in the presence of LPS (10 ng/ml). (C) Kinetics of expression of representative primary response genes in LPS (10 ng/ml)-induced RAW 264.7 macrophages transfected with siControl or SP-siRuvbl2. (D) Kinetics of representative secondary response genes expression in siControl and SP-siRuvbl2 transfected cells in response to LPS (10 ng/ml). Data from (A–D) are obtained from three independent experiments and presented in mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, by unpaired t-test in (B) and by two-way ANOVA with Bonferroni’s multiple comparison test as post-test in (C, D) .

Article Snippet: For immunoblotting and ChIP assay, the following antibodies were used: mouse monoclonal anti-Reptin 52 (RUVBL2) (Santa Cruz), mouse anti-TIP49A (RUVBL1) (Abcam), mouse anti-β-Actin (Sigma-Aldrich), rabbit anti-p38/MAPK (Cell Signaling Technology), rabbit anti-p-p38/MAPK (T180/Y182; Cell Signaling Technology), rabbit anti-p44/42 (ERK1/2; Cell Signaling Technology), rabbit anti-p-p44/42 MAPK (ERK1/2; Thr202/Tyr204 (Cell Signaling Technology), rabbit anti-SAPK/JNK (Cell Signaling Technology), rabbit anti-p-SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology), rabbit anti-IκBα (Cell Signaling Technology), rabbit anti-Stat1 (Cell Signaling Technology), rabbit anti-p-Stat1(S727) (Cell Signaling Technology), rabbit anti-p-Akt (S473) (Cell Signaling Technology), mouse anti-LAMIN B1 (Santa Cruz), mouse anti-α-tubulin (Sigma-Aldrich), rabbit anit-p50 (Abcam), rabbit anti-H3K4Me3 (Cell Signaling Technology), rabbit anti-H4K20me3 (Santa Cruz Biotechnology), and rabbit anti-p50 (Cell Signaling Technology) antibodies.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Transfection, Expressing, Comparison